Paper in Agewandte Chemie International Edition

angewandte chemie int editSurface-Assisted Large-Scale Ordering of DNA Origami Tiles

  1. Ali Aghebat Rafat1,‡,
  2. Dr. Tobias Pirzer1,‡,
  3. Max B. Scheible1,
  4. Anna Kostina1and
  5. Prof. Dr. Friedrich C. Simmel1,2,*

Angew. Chem. Int. Edit., 53, 7665–7668, July 14, 2014, DOI: 10.1002/anie.201403965

The arrangement of DNA-based nanostructures into extended higher order assemblies is an important step towards their utilization as functional molecular materials. We herein demonstrate that by electrostatically controlling the adhesion and mobility of DNA origami structures on mica surfaces by the simple addition of monovalent cations, large ordered 2D arrays of origami tiles can be generated. The lattices can be formed either by close-packing of symmetric, non-interacting DNA origami structures, or by utilizing blunt-end stacking interactions between the origami units. The resulting crystalline lattices can be readily utilized as templates for the ordered arrangement of proteins.