Paper in Angewandte Chemie International Edition

One-Step Formation of “Chain-Armor”-Stabilized DNA Nanostructuresangew chem int ed

Cassinelli V., Oberleitner B., Sobotta J., Nickels P., Grossi G., Kempter S., Frischmuth T., Liedl T., Manetto A.

Angew. Chem. Int. Ed., 2015, May 15, doi: 10.1002/anie.201500561

baseclick GmbH, Bahnhofstrasse 9-15, 82327 Tutzing, Germany

Department Chemistry and Biochemistry, Ludwig-Maximilians-Universität (LMU), Butenandtstrasse 5-13, 81377 Munich, Germany

Department Applied Chemistry, Technische Hochschule Nürnberg G. S. Ohm, Kesslerplatz 12, 90489 Nürnberg, Germany

Physics Department and CeNS, Ludwig-Maximilians-Universität (LMU), Geschwister-Scholl-Platz 1, 80539 Munich, Germany

Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus, Denmark

DNA-based self-assembled nanostructures are widely used to position organic and inorganic objects with nanoscale precision. A particular promising application of DNA structures is their usage as programmable carrier systems for targeted drug delivery. To provide DNA-based templates that are robust against degradation at elevated temperatures, low ion concentrations, adverse pH conditions, and DNases, we built 6-helix DNA tile tubes consisting of 24 oligonucleotides carrying alkyne groups on their 3′-ends and azides on their 5′-ends. By a mild click reaction, the two ends of selected oligonucleotides were covalently connected to form rings and interlocked DNA single strands, so-called DNA catenanes. Strikingly, the structures stayed topologically intact in pure water and even after precipitation from EtOH. The structures even withstood a temperature of 95 °C when all of the 24 strands were chemically interlocked.