Cassinelli V., Oberleitner B., Sobotta J., Nickels P., Grossi G., Kempter S., Frischmuth T., Liedl T., Manetto A.
Angew. Chem. Int. Ed., 2015, May 15, doi: 10.1002/anie.201500561
baseclick GmbH, Bahnhofstrasse 9-15, 82327 Tutzing, Germany
Department Chemistry and Biochemistry, Ludwig-Maximilians-Universität (LMU), Butenandtstrasse 5-13, 81377 Munich, Germany
Department Applied Chemistry, Technische Hochschule Nürnberg G. S. Ohm, Kesslerplatz 12, 90489 Nürnberg, Germany
Physics Department and CeNS, Ludwig-Maximilians-Universität (LMU), Geschwister-Scholl-Platz 1, 80539 Munich, Germany
Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus, Denmark
DNA-based self-assembled nanostructures are widely used to position organic and inorganic objects with nanoscale precision. A particular promising application of DNA structures is their usage as programmable carrier systems for targeted drug delivery. To provide DNA-based templates that are robust against degradation at elevated temperatures, low ion concentrations, adverse pH conditions, and DNases, we built 6-helix DNA tile tubes consisting of 24 oligonucleotides carrying alkyne groups on their 3′-ends and azides on their 5′-ends. By a mild click reaction, the two ends of selected oligonucleotides were covalently connected to form rings and interlocked DNA single strands, so-called DNA catenanes. Strikingly, the structures stayed topologically intact in pure water and even after precipitation from EtOH. The structures even withstood a temperature of 95 °C when all of the 24 strands were chemically interlocked.