Technical University of Munich (TUM) (Germany)

NoTUM - lang positions available.

PROJECT NO. 6 – PhD POSITION

Project title: Single molecule studies of molecular motion and (bio-) chemical reactions on DNA origami platforms.

Project description: The goal of this project is the study of dynamic molecular processes localized on DNA origami platforms. This will initially comprise the study of diffusion of DNA devices, proteins, or nanoparticles on origami structures, and later the realization of directed motion on such structures. A related topic is the study of biochemical reactions in DNA-designed environments. Here, either enzymes or their substrates will be arranged on DNA origami structures and the influence of geometry/confinement/local diffusion on the reactions will be investigated. To study these processes, a combination of AFM, super-resolution microscopy, and other single molecule fluorescence techniques will be employed.

Requirements: The applicant for this project should hold a master (or equivalent) in (bio-)physics or (bio-)chemistry. She/He ideally has previous experience with single molecule techniques and a solid background in statistical physics, reaction kinetics, and stochastic processes.

Contact: Professor Friedrich C. Simmelsimmel@tum.de; Technical University of Munich, Germany

Application deadline: Call is closed

Application: Call is closed

PROJECT NO. 7 – PhD POSITION

Project title: Operation of DNA/RNA nanodevices in living cells.

Project description: The goal of this project is the operation of DNA- or RNA-based nanodevices inside living cells. This can involve the operation of simple DNA switches as biosensors in vivo,  the utilization of DNA nanoconstructs as delivery vehicles, or the production of RNA-based nanodevices in vivo. Depending on the nature of the devices, coupling to a variety of cellular signals will be attempted. Small molecules or ions may be used to actuate aptamers, or induce the formation of special DNA structures. Small RNA molecules may also be used to trigger conformational changes of DNA devices, or induce the opening of DNA-based containers. Readout will be performed using fluorescence techniques, or by coupling the action of the devices to a detectable cellular response, e.g. activation of a reporter gene.

Requirements: The applicant for this project should hold a master (or equivalent) in (bio-)physics or (bio-)chemistry. She/He ideally has some experience with cell-culture, fluorescence microscopic techniques, and genetic engineering.

Contact: Professor Friedrich C. Simmelsimmel@tum.de; Technical University of Munich, Germany

Application deadline: Call is closed

Application site: Call is closed